助教 下平義隆Yoshitaka Shimotai
経歴
平成 9年 岩手県立不来方高等学校卒業
平成13年 青森大学工学部卒業
平成15年 岩手大学大学院農学研究科修了
平成18年 岩手大学大学院連合農学研究科修了
所属学会・学会活動
1.日本ウイルス学会
2.日本分子生物学会
教育専門分野
微生物学（ウイルス学、細菌学）の実習
研究テーマ
1) C型インフルエンザウイルスの増殖機構の解析
2) A型インフルエンザウイルスの感染により発現誘導される宿主遺伝子の機能解析
連絡先
E-mail: yoshimo★med.id.yamagata-u.ac.jp
※「★」を「@」にご変換ください
主な業績
1. Matsuzaki Y, Ohmiya S, Ota R, Kitai Y, Watanabe O, Kitaoka S, Kumaki S, Onuma R, Watanabe Y, Nagai Y, Kadowaki Y, Shimotai Y, Nishimura H. Epidemiologic, clinical, and genetic characteristics of influenza C virus infections among outpatients and inpatients in Sendai, Japan from 2006 to 2020. J Clin Virol. 2023 May;162:105429. doi: 10.1016/j.jcv.2023.105429. Epub 2023 Apr 4. PMID: 37031609.
2. Yamaya M, Deng X, Kikuchi A, Sugawara M, Saito N, Kubo T, Momma H, Kawase T, Nakagome K, Shimotai Y, Nishimura H. The proton ATPase inhibitor bafilomycin A1 reduces the release of rhinovirus C and cytokines from primary cultures of human nasal epithelial cells. Virus Res. 2021 Oct 15;304:198548. doi: 10.1016/j.virusres.2021.198548. Epub 2021 Aug 21. PMID: 34425162.
3. Sato K, Hayashi H, Shimotai Y, Yamaya M, Hongo S, Kawakami K, Matsuzaki Y, Nishimura H. TMPRSS2 Activates Hemagglutinin-Esterase Glycoprotein of Influenza C Virus. J Virol. 2021 Oct 13;95(21):e0129621. doi: 10.1128/JVI.01296-21. Epub 2021 Aug 18. PMID: 34406864; PMCID: PMC8513465.
4. Matsuzaki Y, Sugawara K, Shimotai Y, Kadowaki Y, Hongo S, Mizuta K, Nishimura H. Growth Kinetics of Influenza C Virus Antigenic Mutants That Escaped from Anti-Hemagglutinin Esterase Monoclonal Antibodies and Viral Antigenic Changes Found in Field Isolates. Viruses. 2021 Mar 3;13(3):401. doi: 10.3390/v13030401. PMID: 33802440; PMCID: PMC7998938.
5. Yamaya M, Shimotai Y, Ohkawara A, Bazarragchaa E, Okamatsu M, Sakoda Y, Kida H, Nishimura H. The clinically used serine protease inhibitor nafamostat reduces influenza virus replication and cytokine production in human airway epithelial cells and viral replication in mice. J Med Virol. 2021 Jun;93(6):3484-3495. doi: 10.1002/jmv.26700. Epub 2020 Dec 17. PMID: 33247612; PMCID: PMC7753675.
6. Komabayashi K, Matoba Y, Tanaka S, Seto J, Aoki Y, Ikeda T, Shimotai Y, Matsuzaki Y, Itagaki T, Mizuta K. Longitudinal epidemiology of human coronavirus OC43 in Yamagata, Japan, 2010-2017: Two groups based on spike gene appear one after another. J Med Virol. 2021 Feb;93(2):945-951. doi: 10.1002/jmv.26361. Epub 2020 Aug 13. PMID: 32720708.
7. Matsuzaki Y, Shimotai Y, Kadowaki Y, Sugawara K, Hongo S, Mizuta K, Nishimura H. Antigenic changes among the predominantly circulating C/Sao Paulo lineage strains of influenza C virus in Yamagata, Japan, between 2015 and 2018. Infect Genet Evol. 2020 Jul;81:104269. doi: 10.1016/j.meegid.2020.104269. Epub 2020 Mar 2. PMID: 32135195.
8. Yamaya M, Nishimura H, Deng X, Sugawara M, Watanabe O, Nomura K, Shimotai Y, Momma H, Ichinose M, Kawase T. Inhibitory effects of glycopyrronium, formoterol, and budesonide on coronavirus HCoV-229E replication and cytokine production by primary cultures of human nasal and tracheal epithelial cells. Respir Investig. 2020 May;58(3):155-168. doi: 10.1016/j.resinv.2019.12.005. Epub 2020 Feb 21. PMID: 32094077; PMCID: PMC7102607.
9. Suzuki Y, Seto J, Shimotai Y, Itagaki T, Katsushima Y, Katsushima F, Ikeda T, Mizuta K, Hongo S, Matsuzaki Y. Polyclonal spread of multiple genotypes of Mycoplasma pneumoniae in semi-closed settings in Yamagata, Japan. J Med Microbiol. 2019 May;68(5):785-790. doi: 10.1099/jmm.0.000969. Epub 2019 Apr 1. PMID: 30932805.
10. Yamaya M, Nishimura H, Lusamba Kalonji N, Deng X, Momma H, Shimotai Y, Nagatomi R. Effects of high temperature on pandemic and seasonal human influenza viral replication and infection-induced damage in primary human tracheal epithelial cell cultures. Heliyon. 2019 Feb 5;5(2):e01149. doi: 10.1016/j.heliyon.2019.e01149. PMID: 30839917; PMCID: PMC6365403.
11. Matsuzaki Y, Sugawara K, Furuse Y, Shimotai Y, Hongo S, Mizuta K, Nishimura H. Neutralizing Epitopes and Residues Mediating the Potential Antigenic Drift of the Hemagglutinin-Esterase Protein of Influenza C Virus. Viruses. 2018 Aug 9;10(8):417. doi: 10.3390/v10080417. PMID: 30096880; PMCID: PMC6116000.
12. Matoba Y, Aoki Y, Tanaka S, Unno M, Komabayashi K, Ikeda T, Shimotai Y, Matsuzaki Y, Itagaki T, Mizuta K. Trends of Human Coronaviruses in Yamagata, Japan in 2015-2016 Focusing on the OC43 Outbreak of June 2016. Jpn J Infect Dis. 2018 Mar 22;71(2):167-169. doi: 10.7883/yoken.JJID.2017.263. Epub 2018 Feb 28. PMID: 29491239.
13. Suzuki Y, Seto J, Shimotai Y, Itagaki T, Katsushima Y, Katsushima F, Ikeda T, Mizuta K, Hongo S, Matsuzaki Y. Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Isolates between 2004 and 2014 in Yamagata, Japan: Change in Molecular Characteristics during an 11-year Period. Jpn J Infect Dis. 2017 Nov 22;70(6):642-646. doi: 10.7883/yoken.JJID.2017.276. Epub 2017 Oct 31. PMID: 29093323.
14. Suzuki Y, Shimotai Y, Itagaki T, Seto J, Ikeda T, Yahagi K, Mizuta K, Hongo S, Matsuzaki Y. Development of macrolide resistance-associated mutations after macrolide treatment in children infected with Mycoplasma pneumoniae. J Med Microbiol. 2017 Nov;66(11):1531-1538. doi: 10.1099/jmm.0.000582. Epub 2017 Oct 6. PMID: 28984229.
15. Goto T, Shimotai Y, Matsuzaki Y, Muraki Y, Sho R, Sugawara K, Hongo S. Effect of Phosphorylation of CM2 Protein on Influenza C Virus Replication. J Virol. 2017 Oct 27;91(22):e00773-17. doi: 10.1128/JVI.00773-17. PMID: 28878070; PMCID: PMC5660502.
16. Suzuki Y, Seto J, Shimotai Y, Ikeda T, Yahagi K, Mizuta K, Matsuzaki Y, Hongo S. Development of an endpoint genotyping assay to detect the Mycoplasma pneumoniae 23S rRNA gene and distinguish the existence of macrolide resistance-associated mutations at position 2063. J Microbiol Methods. 2016 Dec;131:130-134. doi: 10.1016/j.mimet.2016.10.017. Epub 2016 Oct 24. PMID: 27789313.
17. Matsuzaki Y, Sugawara K, Furuse Y, Shimotai Y, Hongo S, Oshitani H, Mizuta K, Nishimura H. Genetic Lineage and Reassortment of Influenza C Viruses Circulating between 1947 and 2014. J Virol. 2016 Aug 26;90(18):8251-65. doi: 10.1128/JVI.00969-16. PMID: 27384661; PMCID: PMC5008092.
18. Matoba Y, Aoki Y, Tanaka S, Yahagi K, Shimotai Y, Matsuzaki Y, Itagaki T, Mizuta K. An Outbreak of Human Coronavirus OC43 during the 2014-2015 Influenza Season in Yamagata, Japan. Jpn J Infect Dis. 2015;68(5):442-5. doi: 10.7883/yoken.JJID.2015.292. PMID: 26399927.
19. Yamaya M, Shimotai Y, Hatachi Y, Lusamba Kalonji N, Tando Y, Kitajima Y, Matsuo K, Kubo H, Nagatomi R, Hongo S, Homma M, Nishimura H. The serine protease inhibitor camostat inhibits influenza virus replication and cytokine production in primary cultures of human tracheal epithelial cells. Pulm Pharmacol Ther. 2015 Aug;33:66-74. doi: 10.1016/j.pupt.2015.07.001. Epub 2015 Jul 10. PMID: 26166259; PMCID: PMC7110702.
20. Furukawa T, Shimotai Y, Ohta N, Ishida A, Kurakami K, Suzuki H, Yamakawa M, Hongo S, Kakehata S. Epstein-Barr virus in the enlarged salivary tissues of patients with IgG4-related disease. Laryngoscope. 2015 Sep;125(9):2079-84. doi: 10.1002/lary.25153. Epub 2015 Jul 7. PMID: 26152762.
21. Shimotai Y, Goto T, Matsuzaki Y, Muraki Y, Sugawara K, Hongo S. The effect of the cytoplasmic tail of influenza C virus CM2 protein on its biochemical properties and intracellular processing. Biochem Biophys Rep. 2015 Jul 8;3:1-6. doi: 10.1016/j.bbrep.2015.07.001. PMID: 29124162; PMCID: PMC5668659.
22. Matsuzaki Y, Sugawara K, Nakauchi M, Takahashi Y, Onodera T, Tsunetsugu-Yokota Y, Matsumura T, Ato M, Kobayashi K, Shimotai Y, Mizuta K, Hongo S, Tashiro M, Nobusawa E. Epitope mapping of the hemagglutinin molecule of A/(H1N1)pdm09 influenza virus by using monoclonal antibody escape mutants. J Virol. 2014 Nov;88(21):12364-73. doi: 10.1128/JVI.01381-14. Epub 2014 Aug 13. PMID: 25122788; PMCID: PMC4248900.
23. Matsuzaki Y, Sugawara K, Abiko C, Ikeda T, Aoki Y, Mizuta K, Katsushima N, Katsushima F, Katsushima Y, Itagaki T, Shimotai Y, Hongo S, Muraki Y, Nishimura H. Epidemiological information regarding the periodic epidemics of influenza C virus in Japan (1996-2013) and the seroprevalence of antibodies to different antigenic groups. J Clin Virol. 2014 Sep;61(1):87-93. doi: 10.1016/j.jcv.2014.06.017. Epub 2014 Jun 28. PMID: 25017953.
24. Matsuzaki Y, Ikeda T, Abiko C, Aoki Y, Mizuta K, Shimotai Y, Sugawara K, Hongo S. Detection and quantification of influenza C virus in pediatric respiratory specimens by real-time PCR and comparison with infectious viral counts. J Clin Virol. 2012 Jun;54(2):130-4. doi: 10.1016/j.jcv.2012.02.012. Epub 2012 Mar 6. PMID: 22398038.
25. Furukawa T, Muraki Y, Noda T, Takashita E, Sho R, Sugawara K, Matsuzaki Y, Shimotai Y, Hongo S. Role of the CM2 protein in the influenza C virus replication cycle. J Virol. 2011 Feb;85(3):1322-9. doi: 10.1128/JVI.01367-10. Epub 2010 Nov 24. PMID: 21106743; PMCID: PMC3020500.
26. Yasuda T, Yamaki M, Iimura A, Shimotai Y, Shimizu K, Noshita T, Funayama S. Anti-influenza virus principles from Muehlenbeckia hastulata. J Nat Med. 2010 Apr;64(2):206-11. doi: 10.1007/s11418-009-0386-9. PMID: 20082146.
27. Trinh QD, Izumi Y, Komine-Aizawa S, Shibata T, Shimotai Y, Kuroda K, Mizuguchi M, Ushijima H, Mor G, Hayakawa S. H3N2 influenza A virus replicates in immortalized human first trimester trophoblast cell lines and induces their rapid apoptosis. Am J Reprod Immunol. 2009 Sep;62(3):139-46. doi: 10.1111/j.1600-0897.2009.00723.x. PMID: 19694639; PMCID: PMC3025808.
28. Shimotai Y, Minami H, Saitoh Y, Onodera Y, Mishima Y, Kelm RJ Jr, Tsutsumi K. A binding site for Pur alpha and Pur beta is structurally unstable and is required for replication in vivo from the rat aldolase B origin. Biochem Biophys Res Commun. 2006 Feb 10;340(2):517-25. doi: 10.1016/j.bbrc.2005.12.032. Epub 2005 Dec 19. PMID: 16376299.